Fungal Communities in Canopy Soils

  • Wed Nov 8th, 2017 11:14am
  • News

By Frank Hanson

ONRC – 7 p.m. Nov. 10 –

Evening Talk: Korena Mafune: Updated research on

Old-Growth Temperate Rainforests in Western Washington: Fungal Communities in Canopy Soils as Adaptive Facilitators to

Environmental Change

Korena Mafune received her Bachelor of Science in Restoration Ecology and Environmental Horticulture from the University of Washington in 2013. She has always been passionate about Washington’s beautiful forests and a bit stubborn about staying in her home state.

So directly upon completion of her B.S., she applied and was accepted to UW’s School of Environmental and Forest Sciences (SEFS) graduate program. She completed her M.S. in 2015 and is now in her 2nd year of her PhD research in SEFS. She works closely with Drs. Daniel and Kristina Vogt, who run the interdisciplinary Vogt Lab of Ecosystem and Conservation Ecology.

Her master’s and current PhD research focuses on canopy soils developing on branches of Acer macrophyllum (big leaf maple), located in Washington State’s old-growth temperate rainforests. Her PhD research focuses on fungi associating with adventitious roots growing in the canopy soils of old-growth maple. She is very interested in the potential role fungi may have in canopy soils and the resiliency of these old-growth forests, as we face wetter winters and drier summers. She will be presenting some preliminary results on the diversity of fungi found in these ecosystems, and will also present some soil phosphorus and nitrogen results from her study plots.

For this evening talk series, Korena will discuss her latest research and update us from her past presentation in May of 2017. Her presentation will build from the ground up, as she discusses the life-long mutualistic relationships between plants and fungi, and how they contribute to the unique characteristics of our old-growth temperate rainforests.

Over the past three decades, organic canopy soils and adventitious roots have been reported in temperate old-growth rainforests. Despite these reports, experiments have not elucidated their role in the functioning and resilience of these rainforests to droughts. Evidence shows adventitious roots form fungal mutualists, but not how diversity and/or functional roles vary compared to the forest floors.

The aim of this research is to determine if the community diversity of mutualistic fungi in canopy soils enhance the resilience of Acer macrophyllum to drier climates vs. communities found in the forest floor, and if these communities respond to pulses of phosphorus, in respect to the varying soil characteristics found in canopy vs. forest floor soil ecosystems.

Preliminary research extracted fungal DNA from forest floor and canopy root-tips of old growth Acer macrophyllum located in an old-growth temperate rainforest of Olympic National Park. Sequences suggested fungal diversities differ between canopy and forest floor soils. To test the functional roles of these diverse mutualists, a larger-scale experimental manipulation of water, DNA analyses and imaging is being pursued.

Her research project will experimentally reduce or increase precipitation levels on 18 old-growth Acer macrophyllum trees in two similar temperate rainforest stands. During the experimental drought manipulation, fungal communities from both forest floor and canopy rooting systems will be sequenced and stained for fluorescent microscopy.

Also, changes in available Phosphorous will be assessed in drought to determine if seasonal rainfall regimes and resulting nutrient availabilities are a determining factor in the structure of these fungal communities. This technical description is from Korena Mafune.

Please join us on Nov. 10, Friday night, at 7 p.m. in the Hemlock Forest Room. Evening Talks at ONRC is funded through the Rosmond Forestry Education Fund, an endowment that honors the contributions of Fred Rosmond and his family to forestry and the Forks community. Refreshments will be served and a potluck of your favorite dessert is encouraged. For more information contact Frank Hanson at 360-374-4556 or [email protected]